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brequinar bre sodium  (Tocris)


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    Tocris brequinar bre sodium
    Suppression of GLDC induces dNTPs depletion, resulting in ROS accumulation and leading to mitochondrial stress in RCC cells. (A) Quantification of dTTP, dATP, dCTP, and dGTP levels in ACHN and Caki-2 cells. CTL, control; shGLDC, knockdown of GLDC. Data are shown as the means ± SD ( n =3). (B) Representative micrographs of immunofluorescence staining for mitoSOX with 4',6-diamidino-2-phenylindole (DAPI) counterstaining. ACHN cells were treated with <t>brequinar</t> (Bre) 10 µM for 48 h. ImageJ is used for quantification of the fluorescence intensity. NT, non-treated cells. Data are shown as the means ± SD ( n =3). (C) Representative micrographs of immunofluorescence staining for mitoSOX with DAPI counterstaining in control (CTL) and GLDC knock-downed (shGLDC) cells. The quantifications of fluorescence intensity are on the right side. Data are shown as the means ± SD ( n =3). MitoSOX staining intensity in ACHN CTL and shGLDC cells was confirmed by FACS analysis. Data are shown as the means ± SD ( n =3). (D) Representative micrographs of immunofluorescence staining for mitochondria (anti-HSP60) and mitochondrial (mt) DNA nucleoids (anti-DNA). ImageJ was used to calculate the size of mtDNA nucleoids. Mito, mitochondria. p values were calculated using two-tailed unpaired Student t -test. * p < 0.05, ** p < 0.01, and *** p < 0.001.
    Brequinar Bre Sodium, supplied by Tocris, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/brequinar bre sodium/product/Tocris
    Average 94 stars, based on 11 article reviews
    brequinar bre sodium - by Bioz Stars, 2026-02
    94/100 stars

    Images

    1) Product Images from "Glycine Decarboxylase Regulates Renal Carcinoma Progression via Interferon Stimulated Gene Factor 3-Mediated Pathway"

    Article Title: Glycine Decarboxylase Regulates Renal Carcinoma Progression via Interferon Stimulated Gene Factor 3-Mediated Pathway

    Journal: International Journal of Biological Sciences

    doi: 10.7150/ijbs.104458

    Suppression of GLDC induces dNTPs depletion, resulting in ROS accumulation and leading to mitochondrial stress in RCC cells. (A) Quantification of dTTP, dATP, dCTP, and dGTP levels in ACHN and Caki-2 cells. CTL, control; shGLDC, knockdown of GLDC. Data are shown as the means ± SD ( n =3). (B) Representative micrographs of immunofluorescence staining for mitoSOX with 4',6-diamidino-2-phenylindole (DAPI) counterstaining. ACHN cells were treated with brequinar (Bre) 10 µM for 48 h. ImageJ is used for quantification of the fluorescence intensity. NT, non-treated cells. Data are shown as the means ± SD ( n =3). (C) Representative micrographs of immunofluorescence staining for mitoSOX with DAPI counterstaining in control (CTL) and GLDC knock-downed (shGLDC) cells. The quantifications of fluorescence intensity are on the right side. Data are shown as the means ± SD ( n =3). MitoSOX staining intensity in ACHN CTL and shGLDC cells was confirmed by FACS analysis. Data are shown as the means ± SD ( n =3). (D) Representative micrographs of immunofluorescence staining for mitochondria (anti-HSP60) and mitochondrial (mt) DNA nucleoids (anti-DNA). ImageJ was used to calculate the size of mtDNA nucleoids. Mito, mitochondria. p values were calculated using two-tailed unpaired Student t -test. * p < 0.05, ** p < 0.01, and *** p < 0.001.
    Figure Legend Snippet: Suppression of GLDC induces dNTPs depletion, resulting in ROS accumulation and leading to mitochondrial stress in RCC cells. (A) Quantification of dTTP, dATP, dCTP, and dGTP levels in ACHN and Caki-2 cells. CTL, control; shGLDC, knockdown of GLDC. Data are shown as the means ± SD ( n =3). (B) Representative micrographs of immunofluorescence staining for mitoSOX with 4',6-diamidino-2-phenylindole (DAPI) counterstaining. ACHN cells were treated with brequinar (Bre) 10 µM for 48 h. ImageJ is used for quantification of the fluorescence intensity. NT, non-treated cells. Data are shown as the means ± SD ( n =3). (C) Representative micrographs of immunofluorescence staining for mitoSOX with DAPI counterstaining in control (CTL) and GLDC knock-downed (shGLDC) cells. The quantifications of fluorescence intensity are on the right side. Data are shown as the means ± SD ( n =3). MitoSOX staining intensity in ACHN CTL and shGLDC cells was confirmed by FACS analysis. Data are shown as the means ± SD ( n =3). (D) Representative micrographs of immunofluorescence staining for mitochondria (anti-HSP60) and mitochondrial (mt) DNA nucleoids (anti-DNA). ImageJ was used to calculate the size of mtDNA nucleoids. Mito, mitochondria. p values were calculated using two-tailed unpaired Student t -test. * p < 0.05, ** p < 0.01, and *** p < 0.001.

    Techniques Used: Control, Knockdown, Immunofluorescence, Staining, Fluorescence, Two Tailed Test

    GLDC regulates RCC cell proliferation by activating ISGF3 through the inhibition of dNTP synthesis. (A) Growth curve of ACHN cells treated with brequinar (Bre) 10 µM or without (NT) was assessed by CCK-8 assays. Data are shown as the means ± SD ( n =3). (B) Western blot of indicated proteins in ACHN cells treated with Bre 10 µM or 20 µM for 48 h. (C) qPCR analysis of ISGs in ACHN treated with Bre 10 µM compared to non-treated control cells (NT). Data are shown as the means ± SD ( n =3). (D) Representative micrographs of immunofluorescence staining for mitoSOX in indicated cells with or without the addition of each dNs 10 µM and EHNA 5 µM for 48 h. The fluorescence intensity was calculated by ImageJ ( n =3). (E) Cellular growth of ACHN control (CTL) and GLDC knock-downed cells (shGLDC) after the addition of each dNs 10 µM and EHNA 5 µM to inhibit of degradation of dATP in cell culture. Data are shown as the means ± SD ( n =3). (F) Western blot of indicated proteins in ACHN CTL and shGLDC cells 48 h after the addition of each dNs 10 µM and EHNA 5 µM. p values were calculated using two-tailed unpaired Student t -test. * p < 0.05, ** p < 0.01, and *** p < 0.001.
    Figure Legend Snippet: GLDC regulates RCC cell proliferation by activating ISGF3 through the inhibition of dNTP synthesis. (A) Growth curve of ACHN cells treated with brequinar (Bre) 10 µM or without (NT) was assessed by CCK-8 assays. Data are shown as the means ± SD ( n =3). (B) Western blot of indicated proteins in ACHN cells treated with Bre 10 µM or 20 µM for 48 h. (C) qPCR analysis of ISGs in ACHN treated with Bre 10 µM compared to non-treated control cells (NT). Data are shown as the means ± SD ( n =3). (D) Representative micrographs of immunofluorescence staining for mitoSOX in indicated cells with or without the addition of each dNs 10 µM and EHNA 5 µM for 48 h. The fluorescence intensity was calculated by ImageJ ( n =3). (E) Cellular growth of ACHN control (CTL) and GLDC knock-downed cells (shGLDC) after the addition of each dNs 10 µM and EHNA 5 µM to inhibit of degradation of dATP in cell culture. Data are shown as the means ± SD ( n =3). (F) Western blot of indicated proteins in ACHN CTL and shGLDC cells 48 h after the addition of each dNs 10 µM and EHNA 5 µM. p values were calculated using two-tailed unpaired Student t -test. * p < 0.05, ** p < 0.01, and *** p < 0.001.

    Techniques Used: Inhibition, CCK-8 Assay, Western Blot, Control, Immunofluorescence, Staining, Fluorescence, Cell Culture, Two Tailed Test



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    brequinar bre sodium  (Tocris)
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    Tocris brequinar bre sodium
    Suppression of GLDC induces dNTPs depletion, resulting in ROS accumulation and leading to mitochondrial stress in RCC cells. (A) Quantification of dTTP, dATP, dCTP, and dGTP levels in ACHN and Caki-2 cells. CTL, control; shGLDC, knockdown of GLDC. Data are shown as the means ± SD ( n =3). (B) Representative micrographs of immunofluorescence staining for mitoSOX with 4',6-diamidino-2-phenylindole (DAPI) counterstaining. ACHN cells were treated with <t>brequinar</t> (Bre) 10 µM for 48 h. ImageJ is used for quantification of the fluorescence intensity. NT, non-treated cells. Data are shown as the means ± SD ( n =3). (C) Representative micrographs of immunofluorescence staining for mitoSOX with DAPI counterstaining in control (CTL) and GLDC knock-downed (shGLDC) cells. The quantifications of fluorescence intensity are on the right side. Data are shown as the means ± SD ( n =3). MitoSOX staining intensity in ACHN CTL and shGLDC cells was confirmed by FACS analysis. Data are shown as the means ± SD ( n =3). (D) Representative micrographs of immunofluorescence staining for mitochondria (anti-HSP60) and mitochondrial (mt) DNA nucleoids (anti-DNA). ImageJ was used to calculate the size of mtDNA nucleoids. Mito, mitochondria. p values were calculated using two-tailed unpaired Student t -test. * p < 0.05, ** p < 0.01, and *** p < 0.001.
    Brequinar Bre Sodium, supplied by Tocris, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/brequinar bre sodium/product/Tocris
    Average 94 stars, based on 1 article reviews
    brequinar bre sodium - by Bioz Stars, 2026-02
    94/100 stars
    		  Buy from Supplier
    brequinar sodium  (Bio-Techne corporation)
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    Bio-Techne corporation brequinar sodium
    Suppression of GLDC induces dNTPs depletion, resulting in ROS accumulation and leading to mitochondrial stress in RCC cells. (A) Quantification of dTTP, dATP, dCTP, and dGTP levels in ACHN and Caki-2 cells. CTL, control; shGLDC, knockdown of GLDC. Data are shown as the means ± SD ( n =3). (B) Representative micrographs of immunofluorescence staining for mitoSOX with 4',6-diamidino-2-phenylindole (DAPI) counterstaining. ACHN cells were treated with <t>brequinar</t> (Bre) 10 µM for 48 h. ImageJ is used for quantification of the fluorescence intensity. NT, non-treated cells. Data are shown as the means ± SD ( n =3). (C) Representative micrographs of immunofluorescence staining for mitoSOX with DAPI counterstaining in control (CTL) and GLDC knock-downed (shGLDC) cells. The quantifications of fluorescence intensity are on the right side. Data are shown as the means ± SD ( n =3). MitoSOX staining intensity in ACHN CTL and shGLDC cells was confirmed by FACS analysis. Data are shown as the means ± SD ( n =3). (D) Representative micrographs of immunofluorescence staining for mitochondria (anti-HSP60) and mitochondrial (mt) DNA nucleoids (anti-DNA). ImageJ was used to calculate the size of mtDNA nucleoids. Mito, mitochondria. p values were calculated using two-tailed unpaired Student t -test. * p < 0.05, ** p < 0.01, and *** p < 0.001.
    Brequinar Sodium, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/brequinar sodium/product/Bio-Techne corporation
    Average 90 stars, based on 1 article reviews
    brequinar sodium - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Suppression of GLDC induces dNTPs depletion, resulting in ROS accumulation and leading to mitochondrial stress in RCC cells. (A) Quantification of dTTP, dATP, dCTP, and dGTP levels in ACHN and Caki-2 cells. CTL, control; shGLDC, knockdown of GLDC. Data are shown as the means ± SD ( n =3). (B) Representative micrographs of immunofluorescence staining for mitoSOX with 4',6-diamidino-2-phenylindole (DAPI) counterstaining. ACHN cells were treated with brequinar (Bre) 10 µM for 48 h. ImageJ is used for quantification of the fluorescence intensity. NT, non-treated cells. Data are shown as the means ± SD ( n =3). (C) Representative micrographs of immunofluorescence staining for mitoSOX with DAPI counterstaining in control (CTL) and GLDC knock-downed (shGLDC) cells. The quantifications of fluorescence intensity are on the right side. Data are shown as the means ± SD ( n =3). MitoSOX staining intensity in ACHN CTL and shGLDC cells was confirmed by FACS analysis. Data are shown as the means ± SD ( n =3). (D) Representative micrographs of immunofluorescence staining for mitochondria (anti-HSP60) and mitochondrial (mt) DNA nucleoids (anti-DNA). ImageJ was used to calculate the size of mtDNA nucleoids. Mito, mitochondria. p values were calculated using two-tailed unpaired Student t -test. * p < 0.05, ** p < 0.01, and *** p < 0.001.

    Journal: International Journal of Biological Sciences

    Article Title: Glycine Decarboxylase Regulates Renal Carcinoma Progression via Interferon Stimulated Gene Factor 3-Mediated Pathway

    doi: 10.7150/ijbs.104458

    Figure Lengend Snippet: Suppression of GLDC induces dNTPs depletion, resulting in ROS accumulation and leading to mitochondrial stress in RCC cells. (A) Quantification of dTTP, dATP, dCTP, and dGTP levels in ACHN and Caki-2 cells. CTL, control; shGLDC, knockdown of GLDC. Data are shown as the means ± SD ( n =3). (B) Representative micrographs of immunofluorescence staining for mitoSOX with 4',6-diamidino-2-phenylindole (DAPI) counterstaining. ACHN cells were treated with brequinar (Bre) 10 µM for 48 h. ImageJ is used for quantification of the fluorescence intensity. NT, non-treated cells. Data are shown as the means ± SD ( n =3). (C) Representative micrographs of immunofluorescence staining for mitoSOX with DAPI counterstaining in control (CTL) and GLDC knock-downed (shGLDC) cells. The quantifications of fluorescence intensity are on the right side. Data are shown as the means ± SD ( n =3). MitoSOX staining intensity in ACHN CTL and shGLDC cells was confirmed by FACS analysis. Data are shown as the means ± SD ( n =3). (D) Representative micrographs of immunofluorescence staining for mitochondria (anti-HSP60) and mitochondrial (mt) DNA nucleoids (anti-DNA). ImageJ was used to calculate the size of mtDNA nucleoids. Mito, mitochondria. p values were calculated using two-tailed unpaired Student t -test. * p < 0.05, ** p < 0.01, and *** p < 0.001.

    Article Snippet: Brequinar (Bre) sodium was purchased from Tocris, Bristol, UK.

    Techniques: Control, Knockdown, Immunofluorescence, Staining, Fluorescence, Two Tailed Test

    GLDC regulates RCC cell proliferation by activating ISGF3 through the inhibition of dNTP synthesis. (A) Growth curve of ACHN cells treated with brequinar (Bre) 10 µM or without (NT) was assessed by CCK-8 assays. Data are shown as the means ± SD ( n =3). (B) Western blot of indicated proteins in ACHN cells treated with Bre 10 µM or 20 µM for 48 h. (C) qPCR analysis of ISGs in ACHN treated with Bre 10 µM compared to non-treated control cells (NT). Data are shown as the means ± SD ( n =3). (D) Representative micrographs of immunofluorescence staining for mitoSOX in indicated cells with or without the addition of each dNs 10 µM and EHNA 5 µM for 48 h. The fluorescence intensity was calculated by ImageJ ( n =3). (E) Cellular growth of ACHN control (CTL) and GLDC knock-downed cells (shGLDC) after the addition of each dNs 10 µM and EHNA 5 µM to inhibit of degradation of dATP in cell culture. Data are shown as the means ± SD ( n =3). (F) Western blot of indicated proteins in ACHN CTL and shGLDC cells 48 h after the addition of each dNs 10 µM and EHNA 5 µM. p values were calculated using two-tailed unpaired Student t -test. * p < 0.05, ** p < 0.01, and *** p < 0.001.

    Journal: International Journal of Biological Sciences

    Article Title: Glycine Decarboxylase Regulates Renal Carcinoma Progression via Interferon Stimulated Gene Factor 3-Mediated Pathway

    doi: 10.7150/ijbs.104458

    Figure Lengend Snippet: GLDC regulates RCC cell proliferation by activating ISGF3 through the inhibition of dNTP synthesis. (A) Growth curve of ACHN cells treated with brequinar (Bre) 10 µM or without (NT) was assessed by CCK-8 assays. Data are shown as the means ± SD ( n =3). (B) Western blot of indicated proteins in ACHN cells treated with Bre 10 µM or 20 µM for 48 h. (C) qPCR analysis of ISGs in ACHN treated with Bre 10 µM compared to non-treated control cells (NT). Data are shown as the means ± SD ( n =3). (D) Representative micrographs of immunofluorescence staining for mitoSOX in indicated cells with or without the addition of each dNs 10 µM and EHNA 5 µM for 48 h. The fluorescence intensity was calculated by ImageJ ( n =3). (E) Cellular growth of ACHN control (CTL) and GLDC knock-downed cells (shGLDC) after the addition of each dNs 10 µM and EHNA 5 µM to inhibit of degradation of dATP in cell culture. Data are shown as the means ± SD ( n =3). (F) Western blot of indicated proteins in ACHN CTL and shGLDC cells 48 h after the addition of each dNs 10 µM and EHNA 5 µM. p values were calculated using two-tailed unpaired Student t -test. * p < 0.05, ** p < 0.01, and *** p < 0.001.

    Article Snippet: Brequinar (Bre) sodium was purchased from Tocris, Bristol, UK.

    Techniques: Inhibition, CCK-8 Assay, Western Blot, Control, Immunofluorescence, Staining, Fluorescence, Cell Culture, Two Tailed Test